KMID : 1134820190480090953
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Journal of the Korean Society of Food Science and Nutrition 2019 Volume.48 No. 9 p.953 ~ p.960
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¥á-Glucosidase Inhibitory Activity of Isoflavones and Saponins from Soybean (Glycine max L.) and Comparisons of Their Constituents during Heat Treatments
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Ha Tae-Joung
Park Jae-Eun Kang Beom-Kyu Kim Hong-Sig Shin Sang-Ouk Seo Jeong-Hyun Oh Eun-Young Kim Sung-Up Kwak Do-Yeon
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Abstract
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¥á-Glucosidase (EC 3.2.1.20) is an enzyme that plays a key role in raising blood sugar levels and it is a potential target for designing agents to treat diabetes mellitus. The objective of this study was to investigate the inhibition of ¥á-glucosidase by twenty two isoflavone and soyasaponin derivatives from soybeans (Glycine max L.). Among them, daidzein and genistein inhibited ¥á-glucosidase with an IC50 value of 13.7¡¾2.5 and 8.8¡¾2.6 ¥ìM, respectively. The inhibition kinetics analyzed by a Dixon plot indicate that daidzein and genistein are noncompetitive inhibitors, and the inhibition constant Ki of daidzein and genistein was calculated to be 14.5 and 10.1 ¥ìM, respectively. However, neither glucosides nor malonyl glucosides inhibited ¥á-glucosidase at up to 100 ¥ìM. Moreover, soyasaponin Ba and soyasaponin Bb' showed a significant inhibitory effect with the IC50 of ca. 25 and 32 ¥ìM through simple reversible slow-binding inhibition. By heat treatment (100oC, 20 min), the contents of genistein, daidzein, soyasaponin Ba, soyasaponin Bb and soyasaponin Bb' significantly increased, but the amount of malonyl-isoflavone glycosides and DDMP soyasaponin decreased drastically.
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KEYWORD
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soybean, ¥á-glucosidase, inhibition, isoflavone, soyasaponin
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